pIG-755_HA-GD2-28z_CAR_RFP-JUN
(Plasmid
#207494)
-
PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 207494 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePUC19
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRFP-JUN, HA-GD2-28z_CAR
-
SpeciesH. sapiens (human)
-
Entrez GeneJUN (a.k.a. AP-1, AP1, c-Jun, cJUN, p39)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byHA-GD2-28z CAR sequence was provided by Crystal Mackall/Robbie Majzner.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.07.27.501186 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pIG-755_HA-GD2-28z_CAR_RFP-JUN was a gift from Alexander Marson (Addgene plasmid # 207494 ; http://n2t.net/addgene:207494 ; RRID:Addgene_207494) -
For your References section:
Modular pooled discovery of synthetic knockin sequences to program durable cell therapies. Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A. Cell. 2023 Sep 14;186(19):4216-4234.e33. doi: 10.1016/j.cell.2023.08.013. 10.1016/j.cell.2023.08.013 PubMed 37714135