pEGFP-C1-hArfaptin1
(Plasmid
#206404)
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PurposeMammalian expression of hArfaptin1
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArfaptin1
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SpeciesH. sapiens (human)
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Entrez GeneARFIP1 (a.k.a. HSU52521)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-hArfaptin1 was a gift from Kazuhisa Nakayama & Hye-Won Shin (Addgene plasmid # 206404 ; http://n2t.net/addgene:206404 ; RRID:Addgene_206404) -
For your References section:
Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking. Shin HW, Kobayashi H, Kitamura M, Waguri S, Suganuma T, Uchiyama Y, Nakayama K. J Cell Sci. 2005 Sep 1;118(Pt 17):4039-48. doi: 10.1242/jcs.02524. 10.1242/jcs.02524 PubMed 16129887