pDisplay-SNAPtag
(Plasmid
#205094)
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PurposeDisplay SNAPtag on the surface of mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205094 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV(MinDis) [variant of pDisplay]
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 5832
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Modifications to backboneFrom pDisplay: Hemagglutinin (HA) tag removed
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAPtag
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Insert Size (bp)546
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer GTG ACA GAT CTG CCG CAG GCA GCA TGG ACA AAG ACT GCG AAA TGA AGC
- 3′ sequencing primer TGT TCG TCG ACC TGC AGC GAG CTG CCC AGC CCA G (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe plasmid backbone was amplified from pCMV(MinDis).iGluSnFR, a gift from Loren Looger (Addgene plasmid # 41732).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDisplay-SNAPtag was a gift from Bianxiao Cui (Addgene plasmid # 205094 ; http://n2t.net/addgene:205094 ; RRID:Addgene_205094) -
For your References section:
Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions. Zhang W, Lu CH, Nakamoto ML, Tsai CT, Roy AR, Lee CE, Yang Y, Jahed Z, Li X, Cui B. Nat Cell Biol. 2023 Sep 28. doi: 10.1038/s41556-023-01238-1. 10.1038/s41556-023-01238-1 PubMed 37770566