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Addgene

pDisplay-SNAPtag
(Plasmid #205094)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 205094 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV(MinDis) [variant of pDisplay]
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 5832
  • Modifications to backbone
    From pDisplay: Hemagglutinin (HA) tag removed
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNAPtag
  • Insert Size (bp)
    546

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GTG ACA GAT CTG CCG CAG GCA GCA TGG ACA AAG ACT GCG AAA TGA AGC
  • 3′ sequencing primer TGT TCG TCG ACC TGC AGC GAG CTG CCC AGC CCA G
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The plasmid backbone was amplified from pCMV(MinDis).iGluSnFR, a gift from Loren Looger (Addgene plasmid # 41732).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-SNAPtag was a gift from Bianxiao Cui (Addgene plasmid # 205094 ; http://n2t.net/addgene:205094 ; RRID:Addgene_205094)
  • For your References section:

    Curved adhesions mediate cell attachment to soft matrix fibres in three dimensions. Zhang W, Lu CH, Nakamoto ML, Tsai CT, Roy AR, Lee CE, Yang Y, Jahed Z, Li X, Cui B. Nat Cell Biol. 2023 Sep 28. doi: 10.1038/s41556-023-01238-1. 10.1038/s41556-023-01238-1 PubMed 37770566