pBsVchCAST-pyrE
(Plasmid
#203813)
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PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHT43
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Vector typeBacterial Expression, CRISPR
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Selectable markersChloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
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SpeciesVibrio cholera
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid can also be transformed in Bacillus subtilis, grown at 37 C
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBsVchCAST-pyrE was a gift from Sheng Yang (Addgene plasmid # 203813 ; http://n2t.net/addgene:203813 ; RRID:Addgene_203813)