Skip to main content
Addgene

pET21a- hPTP1e-PDZ2
(Plasmid #20377)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    hPTP1e PDZ2
  • Alt name
    hPTP1e
  • Alt name
    PTP-BAS PDZ2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    288
  • GenBank ID
    XM_172831
  • Entrez Gene
    PTPN13 (a.k.a. FAP-1, PNP1, PTP-BAS, PTP-BL, PTP1E, PTPL1, PTPLE, hPTP1E)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    a gift from Dr David Siderovski (UNC pharmacology)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IMPORTANT: Please note that the His tag in this Pet28 vector is NOT in frame with the Eglin C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a- hPTP1e-PDZ2 was a gift from Andrew Lee (Addgene plasmid # 20377 ; http://n2t.net/addgene:20377 ; RRID:Addgene_20377)

  • For your References section:

    Increased rigidity of eglin c at acidic pH: evidence from NMR spin relaxation and MD simulations. Hu H, Clarkson MW, Hermans J, Lee AL. Biochemistry. 2003 Dec 2. 42(47):13856-68. 10.1021/bi035015z PubMed 14636053
Related items:
Commonly requested with: