pcDNA3.1-FLT3-V5/HIS
(Plasmid
#201985)
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Purposeexpression of human FLT3 receptor tyrosine kinase in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 201985 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1-V5/His
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLT3 receptor tyrosine kinase
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SpeciesH. sapiens (human)
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GenBank IDKAI2569012.1
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Entrez GeneFLT3 (a.k.a. CD135, FLK-2, FLK2, STK1)
- Promoter CMV
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Tag
/ Fusion Protein
- V5, 6xHis (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-FLT3-V5/HIS was a gift from Pavel Krejčí (Addgene plasmid # 201985 ; http://n2t.net/addgene:201985 ; RRID:Addgene_201985) -
For your References section:
One reporter for in-cell activity profiling of majority of protein kinase oncogenes. Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova Bosakova M, Trantirek L, Mayer J, Krejci P. Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536. 10.7554/eLife.21536 PubMed 28199182