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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20131 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
- Backbone size w/o insert (bp) 4835
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeroGFP
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Alt namekar2ss-roGFP2-HDEL
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Alt nameKAR2
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SpeciesS. cerevisiae (budding yeast); jellyfish
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Insert Size (bp)1828
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Entrez GeneKAR2 (a.k.a. YJL034W, GRP78)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byS. James Remington University of Oregon
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPM28 (eroGFP CEN/ARS URA3) was a gift from Feroz Papa (Addgene plasmid # 20131 ; http://n2t.net/addgene:20131 ; RRID:Addgene_20131) -
For your References section:
Real-time redox measurements during endoplasmic reticulum stress reveal interlinked protein folding functions. Merksamer PI, Trusina A, Papa FR. Cell. 2008 Nov 28. 135(5):933-47. 10.1016/j.cell.2008.10.011 PubMed 19026441