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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19937 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-myc3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5486
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Vector typeMammalian Expression
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Selectable markersG418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDKN1B cyclin-dependent kinase inhibitor 1B
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Alt namep27
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Alt nameKip1
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SpeciesH. sapiens (human)
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Insert Size (bp)597
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GenBank IDENST00000228872
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Entrez GeneCDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
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Tag
/ Fusion Protein
- myc3 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Data not available (not destroyed)
- 3′ cloning site Data not available (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-myc3-p27 was a gift from Yue Xiong (Addgene plasmid # 19937 ; http://n2t.net/addgene:19937 ; RRID:Addgene_19937) -
For your References section:
Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. Zariwala M, Liu J, Xiong Y. Oncogene. 1998 Nov 26. 17(21):2787-98. 10.1038/sj.onc.1202505 PubMed 9840943