pCMV-BACH1t_RFP
(Plasmid
#199214)
-
PurposeBACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 199214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneLPUtopia-7
-
Backbone manufacturerBalazsi Lab
- Backbone size w/o insert (bp) 4139
- Total vector size (bp) 9832
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameBACH1t
-
Alt nameBTB domain and CNC homolog 1, transcript variant t
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1872
-
GenBank IDNR_027655.3
-
Entrez GeneBACH1 (a.k.a. BACH-1, BTBD24)
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer gacattgattattgactagt
- 3′ sequencing primer AAACAGTAAgcgactctaga (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameRFP
-
Alt nameRFP
-
SpeciesSynthetic
- Promoter PGK
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer AAGAAGCCCGTGgggtag
- 3′ sequencing primer cagggtaattcgaatttaaa (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-BACH1t_RFP was a gift from Gabor Balazsi (Addgene plasmid # 199214 ; http://n2t.net/addgene:199214 ; RRID:Addgene_199214) -
For your References section:
Nonmonotone invasion landscape by noise-aware control of metastasis activator levels. Wan Y, Cohen J, Szenk M, Farquhar KS, Coraci D, Krzyszton R, Azukas J, Van Nest N, Smashnov A, Chern YJ, De Martino D, Nguyen LC, Bien H, Bravo-Cordero JJ, Chan CH, Rosner MR, Balazsi G. Nat Chem Biol. 2023 Jul;19(7):887-899. doi: 10.1038/s41589-023-01344-z. Epub 2023 May 25. 10.1038/s41589-023-01344-z PubMed 37231268