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Addgene

pET-hMICU1EF
(Plasmid #199180)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 199180 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET24

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MICU1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2450
  • Mutation
    D421A, E432K, F433W, F453W
  • Entrez Gene
    MICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
  • Tags / Fusion Proteins
    • His6 (C terminal on insert)
    • MBP, Thrombin site, TEV site (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-hMICU1EF was a gift from Ming-Feng Tsai (Addgene plasmid # 199180 ; http://n2t.net/addgene:199180 ; RRID:Addgene_199180)
  • For your References section:

    Evidence supporting the MICU1 occlusion mechanism and against the potentiation model in the mitochondrial calcium uniporter complex. Tsai CW, Liu TY, Chao FY, Tu YC, Rodriguez MX, Van Keuren AM, Ma Z, Bankston J, Tsai MF. Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2217665120. doi: 10.1073/pnas.2217665120. Epub 2023 Apr 10. 10.1073/pnas.2217665120 PubMed 37036971