pET-hMICU1EF
(Plasmid
#199180)
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PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199180 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMICU1
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SpeciesH. sapiens (human)
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Insert Size (bp)2450
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MutationD421A, E432K, F433W, F453W
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Entrez GeneMICU1 (a.k.a. CALC, CBARA1, EFHA3, MPXPS, ara CALC)
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Tags
/ Fusion Proteins
- His6 (C terminal on insert)
- MBP, Thrombin site, TEV site (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-hMICU1EF was a gift from Ming-Feng Tsai (Addgene plasmid # 199180 ; http://n2t.net/addgene:199180 ; RRID:Addgene_199180) -
For your References section:
Evidence supporting the MICU1 occlusion mechanism and against the potentiation model in the mitochondrial calcium uniporter complex. Tsai CW, Liu TY, Chao FY, Tu YC, Rodriguez MX, Van Keuren AM, Ma Z, Bankston J, Tsai MF. Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2217665120. doi: 10.1073/pnas.2217665120. Epub 2023 Apr 10. 10.1073/pnas.2217665120 PubMed 37036971