pGL2_173
(Plasmid
#199105)
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PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonen/a
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Gentamicin, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli pir-116
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT
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SpeciesSynthetic
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL2_173 was a gift from Charlie Gilbert & Nili Ostrov & Cultivarium Tools (Addgene plasmid # 199105 ; http://n2t.net/addgene:199105 ; RRID:Addgene_199105) -
For your References section:
A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria. Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N. bioRxiv 2023.05.19.541510 10.1101/2023.05.19.541510