pGL0_22 [pSG5 ORI]
(Plasmid
#198935)
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PurposeLevel 0 part
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 198935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGLDV_17
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)E. coli Zymo 10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepSG5 ORI
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SpeciesStreptomyces ghanaensis
Cloning Information
- Cloning method Unknown
Resource Information
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A portion of this plasmid was derived from a plasmid made byGünther Muth (Muth 2018 https://doi.org/10.1007/s00253-018-9334-5)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL0_22 [pSG5 ORI] was a gift from Charlie Gilbert & Nili Ostrov & Cultivarium Tools (Addgene plasmid # 198935 ; http://n2t.net/addgene:198935 ; RRID:Addgene_198935) -
For your References section:
A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria. Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N. bioRxiv 2023.05.19.541510 10.1101/2023.05.19.541510