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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C2
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameformin1 iso1b
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Alt nameFmn1
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SpeciesM. musculus (mouse)
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Insert Size (bp)4500
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GenBank IDNM_010230
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Entrez GeneFmn1 (a.k.a. RP23-248J10.1, Fmn, ld)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SmaI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC2-FmnIso1b was a gift from Philip Leder (Addgene plasmid # 19320 ; http://n2t.net/addgene:19320 ; RRID:Addgene_19320) -
For your References section:
Formin-1 protein associates with microtubules through a peptide domain encoded by exon-2. Zhou F, Leder P, Martin SS. Exp Cell Res. 2006 Apr 15. 312(7):1119-26. 10.1016/j.yexcr.2005.12.035 PubMed 16480715
Map uploaded by the depositor.