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Addgene

pBabepuro-PDGFB
(Plasmid #189611)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189611 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabepuro
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDGFB
  • Species
    H. sapiens (human)
  • Entrez Gene
    PDGFB (a.k.a. IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis)
  • Promoter SV40 early promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH2 (unknown if destroyed)
  • 3′ cloning site Sal2 (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human U2OS osteosarcoma cells

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabepuro-PDGFB was a gift from Thierry Tchenio (Addgene plasmid # 189611 ; http://n2t.net/addgene:189611 ; RRID:Addgene_189611)