pBBR1_DVS_AH
(Plasmid
#188952)
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Purpose(Empty Backbone) Level 2 destination vector containing pBBR1 origin of replication and CIDAR AH scars
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 188952 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBBR1
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Modifications to backboneCIDAR MoClo cloning site, spectinomycin resistance
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsContains lacZ-alpha cassette for blue/white screening
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBBR1_DVS_AH was a gift from Eric Young (Addgene plasmid # 188952 ; http://n2t.net/addgene:188952 ; RRID:Addgene_188952) -
For your References section:
Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria. Keating KW, Young EM. ACS Synth Biol. 2023 Jun 9. doi: 10.1021/acssynbio.3c00104. 10.1021/acssynbio.3c00104 PubMed 37294017