Dr-FGFR1
(Plasmid
#184005)
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PurposeDr-FGFR expression in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDr-FGFR1
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SpeciesH. sapiens (human)
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Entrez GeneFGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
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Tag
/ Fusion Protein
- Deinococcus radiodurans bacterial phytochrome (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dr-FGFR1 was a gift from Vladislav Verkhusha (Addgene plasmid # 184005 ; http://n2t.net/addgene:184005 ; RRID:Addgene_184005) -
For your References section:
Bacterial Phytochrome as a Scaffold for Engineering of Receptor Tyrosine Kinases Controlled with Near-Infrared Light. Leopold AV, Pletnev S, Verkhusha VV. J Mol Biol. 2020 Jun 12;432(13):3749-3760. doi: 10.1016/j.jmb.2020.04.005. Epub 2020 Apr 14. 10.1016/j.jmb.2020.04.005 PubMed 32302608