FRAP
(Plasmid
#1827)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 1827 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAPtag-1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFR1 AP
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Alt namemFR1
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Alt namemurine FGF receptor 1
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Alt nameFgfr1
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SpeciesM. musculus (mouse)
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Insert Size (bp)3300
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Entrez GeneFgfr1 (a.k.a. Eask, FGFR-I, FLG, Fgfr-1, Flt-2, Fr1, Hspy, MFR, bFGF-R-1, c-fgr)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site See map (unknown if destroyed)
- 3′ cloning site See map (unknown if destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See scanned map.
(Leder #C-281)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRAP was a gift from Philip Leder (Addgene plasmid # 1827 ; http://n2t.net/addgene:1827 ; RRID:Addgene_1827) -
For your References section:
Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells. Ornitz DM, Yayon A, Flanagan JG, Svahn CM, Levi E, Leder P. Mol Cell Biol 1992 Jan;12(1):240-7. PubMed 1309590