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Addgene

FRAP
(Plasmid #1827)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1827 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    APtag-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FR1 AP
  • Alt name
    mFR1
  • Alt name
    murine FGF receptor 1
  • Alt name
    Fgfr1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3300
  • Entrez Gene
    Fgfr1 (a.k.a. Eask, FGFR-I, FLG, Fgfr-1, Flt-2, Fr1, Hspy, MFR, bFGF-R-1, c-fgr)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (unknown if destroyed)
  • 3′ cloning site See map (unknown if destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See scanned map.
(Leder #C-281)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRAP was a gift from Philip Leder (Addgene plasmid # 1827 ; http://n2t.net/addgene:1827 ; RRID:Addgene_1827)
  • For your References section:

    Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells. Ornitz DM, Yayon A, Flanagan JG, Svahn CM, Levi E, Leder P. Mol Cell Biol 1992 Jan;12(1):240-7. PubMed 1309590