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Addgene

Flag-SIRT1
(Plasmid #1791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECE
  • Backbone size w/o insert (bp) 2877
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIRT1
  • Alt name
    Sir2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2314
  • GenBank ID
    NM_012238 NP_036370
  • Entrez Gene
    SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SV40pro-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HindIII site used for cloning this plasmid is not unique. There is an additional HindIII site at nucleotide 1569 of the NCBI Reference Sequence: NM_012238.4. There is a unique SacI site at the 5' end of the insert that could be used for cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-SIRT1 was a gift from Michael Greenberg (Addgene plasmid # 1791 ; http://n2t.net/addgene:1791 ; RRID:Addgene_1791)

  • For your References section:

    Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase. Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, Lin Y, Tran H, Ross SE, Mostoslavsky R, Cohen HY, Hu LS, Cheng HL, Jedrychowski MP, Gygi SP, Sinclair DA, Alt FW, Greenberg ME. Science 2004 Mar 26;303(5666):2011-5. Epub 2004 Feb 19. 10.1126/science.1094637 PubMed 14976264
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