pCAG-mcherry-WIPI2d-cs-TEV-STREP
(Plasmid
#178912)
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PurposeMammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWIPI2d
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SpeciesH. sapiens (human)
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Tags
/ Fusion Proteins
- N term mcherry (N terminal on insert)
- C term strep (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (unknown if destroyed)
- 3′ cloning site XmaI (unknown if destroyed)
- 5′ sequencing primer pCAG for
- 3′ sequencing primer pCAG Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-mcherry-WIPI2d-cs-TEV-STREP was a gift from James Hurley (Addgene plasmid # 178912 ; http://n2t.net/addgene:178912 ; RRID:Addgene_178912) -
For your References section:
Structural basis for membrane recruitment of ATG16L1 by WIPI2 in autophagy. Strong LM, Chang C, Riley JF, Boecker CA, Flower TG, Buffalo CZ, Ren X, Stavoe AK, Holzbaur EL, Hurley JH. Elife. 2021 Sep 10;10. pii: 70372. doi: 10.7554/eLife.70372. 10.7554/eLife.70372 PubMed 34505572
