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Addgene

pPBpuro-miRFP670-HRasCT
(Plasmid #176030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBpuro
  • Backbone size w/o insert (bp) 6900
  • Total vector size (bp) 7931
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Membrane-targeted miRFP670
  • Species
    Synthetic

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBpuro-miRFP670-HRasCT was a gift from Michiyuki Matsuda (Addgene plasmid # 176030 ; http://n2t.net/addgene:176030 ; RRID:Addgene_176030)

  • For your References section:

    A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M. Dev Cell. 2022 Oct 10;57(19):2290-2304.e7. doi: 10.1016/j.devcel.2022.09.003. Epub 2022 Sep 28. 10.1016/j.devcel.2022.09.003 PubMed 36174555
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