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Addgene

Agrp-pGL3
(Plasmid #17552)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17552 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL3
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Agrp
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    396
  • Mutation
    Contains ~400bp of the mouse Pomc gene promoter, including the STAT3/Foxo binding site
  • Entrez Gene
    Agrp (a.k.a. Agrt, Art)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer ?
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Agrp-pGL3 was a gift from Domenico Accili (Addgene plasmid # 17552 ; http://n2t.net/addgene:17552 ; RRID:Addgene_17552)
  • For your References section:

    Forkhead protein FoxO1 mediates Agrp-dependent effects of leptin on food intake. Kitamura T, Feng Y, Kitamura YI, Chua SC, Xu AW, Barsh GS, Rossetti L, Accili D. Nat Med. 2006 May . 12(5):534-40. 10.1038/nm1392 PubMed 16604086