Halo-WIPI2B H85E
(Plasmid #175027)

• Purpose: Expresses WIPI2B (H85E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1
• Depositing Lab: Andrea Stavoe
• Publication: Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi: 10.7554/eLife.70372.

Backbone

• Vector backbone: pHTN
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 6143
• Total vector size (bp): 7410
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: WIPI2B (H85E)
• Species: H. sapiens (human)
• Mutation: Histadine 85 to Glutamate (CAC to GAG)
• Entrez Gene: WIPI2 (a.k.a. ATG18B, Atg21, CGI-50, IDDSSA, WIPI-2)
• Promoter: CMV
• Tag / Fusion Protein:
  • HaloTag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: cctgatcggcagcgagatcg

Resource Information

• Supplemental Documents:
  • halo-wipi2b-h85e.gb
• A portion of this plasmid was derived from a plasmid made by: Sharon Tooze, Francis Crick Institute, UK

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry