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Purpose3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17481 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 8052
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3, 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameenhanced green fluorescent protein
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Alt nameGFP
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Insert Size (bp)719
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byeGFP gene is derived from peGFP-C1 plasmid (Clontech).
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the full sequence was assembled and may therefore contain sequence discrepancies within the backbone that do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV/TO eGFP Puro (w159-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17481 ; http://n2t.net/addgene:17481 ; RRID:Addgene_17481) -
For your References section:
A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394