pSA293-CHEQ-seq
(Plasmid
#174669)
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Purpose(Empty Backbone) Reporter plasmid for MPRA containing a SCP1 promoter, a chimeric intron and the Venus cDNA.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.10
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Backbone manufacturerPromega
- Backbone size (bp) 3730
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Modifications to backboneThe Luciferase gene has been replaced by an expression cassette containing the SCP1 minimal promoter, a chimeric intron and the Venus cDNA. An attB site has been inserted between the SV40 polyA and the ColE1 ori.
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Vector typeEnhancer reporter
- Promoter SCP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSA293-CHEQ-seq was a gift from Stein Aerts (Addgene plasmid # 174669 ; http://n2t.net/addgene:174669 ; RRID:Addgene_174669) -
For your References section:
Analysis of long and short enhancers in melanoma cell states. Mauduit D, Taskiran II, Minnoye L, de Waegeneer M, Christiaens V, Hulselmans G, Demeulemeester J, Wouters J, Aerts S. Elife. 2021 Dec 7;10. pii: 71735. doi: 10.7554/eLife.71735. 10.7554/eLife.71735 PubMed 34874265