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Addgene

pSA293-CHEQ-seq
(Plasmid #174669)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174669 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL4.10
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 3730
  • Modifications to backbone
    The Luciferase gene has been replaced by an expression cassette containing the SCP1 minimal promoter, a chimeric intron and the Venus cDNA. An attB site has been inserted between the SV40 polyA and the ColE1 ori.
  • Vector type
    Enhancer reporter
  • Promoter SCP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSA293-CHEQ-seq was a gift from Stein Aerts (Addgene plasmid # 174669 ; http://n2t.net/addgene:174669 ; RRID:Addgene_174669)
  • For your References section:

    Analysis of long and short enhancers in melanoma cell states. Mauduit D, Taskiran II, Minnoye L, de Waegeneer M, Christiaens V, Hulselmans G, Demeulemeester J, Wouters J, Aerts S. Elife. 2021 Dec 7;10. pii: 71735. doi: 10.7554/eLife.71735. 10.7554/eLife.71735 PubMed 34874265