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PurposeExpresses 150 kD Penn State ladder protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173836 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepST50Tr
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSTRHSTPABMBPpepN
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SpeciesSynthetic
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BsrGI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSU150 was a gift from Song Tan (Addgene plasmid # 173836 ; http://n2t.net/addgene:173836 ; RRID:Addgene_173836) -
For your References section:
The Penn State Protein Ladder system for inexpensive protein molecular weight markers. Santilli RT, Williamson JE 3rd, Shibata Y, Sowers RP, Fleischman AN, Tan S. Sci Rep. 2021 Aug 18;11(1):16703. doi: 10.1038/s41598-021-96051-x. 10.1038/s41598-021-96051-x PubMed 34408191