pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro
(Plasmid
#173232)
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Purpose(Empty Backbone) One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 173232 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NLS_N136aa_ccdb_C21aa_DddA_G1333C__UGI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro was a gift from Bin Shen (Addgene plasmid # 173232 ; http://n2t.net/addgene:173232 ; RRID:Addgene_173232) -
For your References section:
Precision modeling of mitochondrial diseases in zebrafish via DdCBE-mediated mtDNA base editing. Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, Shen B. Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9. 10.1038/s41421-021-00307-9 PubMed 34480028