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PurposeDetect the real-time ER calcium store and local calcium microdomains in live cells.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 172659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunspecified
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecalmodulin
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SpeciesH. sapiens (human)
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MutationPlease see depositor comments
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there is a mixed population of a single base pair in the SNAP tag that could affect the amino acid at position 137. If base is a T, SNAP tag will contain a N137Y mutation. Dep. confirms mutation does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GCEPIA1-SNAPER was a gift from Pingyong Xu (Addgene plasmid # 172659 ; http://n2t.net/addgene:172659 ; RRID:Addgene_172659) -
For your References section:
A genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic reticulum. Luo C, Wang H, Liu Q, He W, Yuan L, Pingyong Xu P. Biophys Rep (2019) 5, 31–42 10.1007/s41048-019-0082-6