AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
(Plasmid
#171596)
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PurposeEctodomain deleted negative control ACE2 from Bxb1 landing pad
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 171596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Vector typeMammalian Expression, Synthetic Biology ; Promoterless Bxb1 DNA recombination
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameACE2
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SpeciesH. sapiens (human)
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Entrez GeneACE2 (a.k.a. ACEH)
Gene/Insert 2
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Gene/Insert namemCherry
Gene/Insert 3
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Gene/Insert nameH2A
Gene/Insert 4
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Gene/Insert namePac
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 171596 ; http://n2t.net/addgene:171596 ; RRID:Addgene_171596) -
For your References section:
Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection. Shukla N, Roelle SM, Suzart VG, Bruchez AM, Matreyek KA. PLoS Pathog. 2021 Jul 16;17(7):e1009715. doi: 10.1371/journal.ppat.1009715. eCollection 2021 Jul. 10.1371/journal.ppat.1009715 PubMed 34270613