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Addgene

pBR-Lent-STAT5A-CA
(Plasmid #170662)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170662 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTRIPZ
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAT5A
  • Species
    H. sapiens (human)
  • Mutation
    H298R and S710F
  • Entrez Gene
    STAT5A (a.k.a. MGF, STAT5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A constitutively active form of STAT5A was cloned into the lentiviral expression vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR-Lent-STAT5A-CA was a gift from Alexander Ruthenburg (Addgene plasmid # 170662 ; http://n2t.net/addgene:170662 ; RRID:Addgene_170662)
  • For your References section:

    Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia. Richter WF, Shah RN, Ruthenburg AJ. Elife. 2021 Jul 15;10. pii: 64960. doi: 10.7554/eLife.64960. 10.7554/eLife.64960 PubMed 34263728