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PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
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Backbone manufacturerJoachim Messing
- Backbone size w/o insert (bp) 2712
- Total vector size (bp) 3991
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
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SpeciesSynthetic
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Insert Size (bp)1279
- Promoter LacI-Plac
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Tags
/ Fusion Proteins
- His-tag (N terminal on insert)
- VHH Amy (N terminal on insert)
- HA-tag (N terminal on insert)
- Prescission site (N terminal on insert)
- E-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site N/A (unknown if destroyed)
- 3′ cloning site N/A (unknown if destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEHlyA5 was a gift from Luis Ángel Fernández (Addgene plasmid # 168298 ; http://n2t.net/addgene:168298 ; RRID:Addgene_168298) -
For your References section:
Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Ruano-Gallego D, Fraile S, Gutierrez C, Fernandez LA. Microb Cell Fact. 2019 Mar 11;18(1):47. doi: 10.1186/s12934-019-1094-0. 10.1186/s12934-019-1094-0 PubMed 30857538