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Addgene

pEHlyA5
(Plasmid #168298)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168298 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC18
  • Backbone manufacturer
    Joachim Messing
  • Backbone size w/o insert (bp) 2712
  • Total vector size (bp) 3991
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
  • Species
    Synthetic
  • Insert Size (bp)
    1279
  • Promoter LacI-Plac
  • Tags / Fusion Proteins
    • His-tag (N terminal on insert)
    • VHH Amy (N terminal on insert)
    • HA-tag (N terminal on insert)
    • Prescission site (N terminal on insert)
    • E-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEHlyA5 was a gift from Luis Ángel Fernández (Addgene plasmid # 168298 ; http://n2t.net/addgene:168298 ; RRID:Addgene_168298)
  • For your References section:

    Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system. Ruano-Gallego D, Fraile S, Gutierrez C, Fernandez LA. Microb Cell Fact. 2019 Mar 11;18(1):47. doi: 10.1186/s12934-019-1094-0. 10.1186/s12934-019-1094-0 PubMed 30857538