pPM1003
(Plasmid
#167550)
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PurposeEncodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHC79
- Backbone size w/o insert (bp) 6000
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Vector typeCosmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVibrio cholerae O antigens genes of the Ogawa serotype
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Alt nameCosmid clone
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SpeciesVibrio cholerae
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPM1003 was a gift from Renato Morona (Addgene plasmid # 167550 ; http://n2t.net/addgene:167550 ; RRID:Addgene_167550) -
For your References section:
Molecular cloning and expression in Escherichia coli K-12 of the O antigens of the Inaba and Ogawa serotypes of the Vibrio cholerae O1 lipopolysaccharides and their potential for vaccine development. Manning PA, Heuzenroeder MW, Yeadon J, Leavesley DI, Reeves PR, Rowley D. Infect Immun. 1986 Aug;53(2):272-7. doi: 10.1128/IAI.53.2.272-277.1986. 10.1128/IAI.53.2.272-277.1986 PubMed 2426198