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Addgene

pcDNA3.1/ZMYM2-FGFR1
(Plasmid #167486)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167486 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 9497
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZMYM2-FGFR1
  • Alt name
    ZNF198-FGFR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4075
  • GenBank ID
    NM_003453.6 NM_023110.3
  • Entrez Gene
    FGFR1 (a.k.a. BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1)
  • Entrez Gene
    ZMYM2 (a.k.a. FIM, MYM, NECRC, RAMP, SCLL, ZNF198)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHl (not destroyed)
  • 3′ cloning site BamHl (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/ZMYM2-FGFR1 was a gift from Nicholas Cross (Addgene plasmid # 167486 ; http://n2t.net/addgene:167486 ; RRID:Addgene_167486)
  • For your References section:

    ZNF198-FGFR1 transforms Ba/F3 cells to growth factor independence and results in high level tyrosine phosphorylation of STATS 1 and 5. Smedley D, Demiroglu A, Abdul-Rauf M, Heath C, Cooper C, Shipley J, Cross NC. Neoplasia. 1999 Oct;1(4):349-55. doi: 10.1038/sj.neo.7900035. 10.1038/sj.neo.7900035 PubMed 10935490