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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
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Vector typexenopus/mammalian/avian/zebrafish
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDkk1
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Alt nameDickkopf-1
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SpeciesH. sapiens (human)
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Entrez GeneDKK1 (a.k.a. DKK-1, SK)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is wild-type, full-length Dickkopf-1, Flag tagged.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+ Dkk1-flag was a gift from Xi He (Addgene plasmid # 16690 ; http://n2t.net/addgene:16690 ; RRID:Addgene_16690) -
For your References section:
Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6. Semënov MV, Tamai K, Brott BK, Kuhl M, Sokol S, He X. Curr Biol. 2001 Jun 26. 11(12):951-61. 10.1016/S0960-9822(01)00290-1 PubMed 11448771