MSCVpuro-6xHis_SUMO2
(Plasmid
#164938)
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PurposeExpresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCVpuro
- Backbone size w/o insert (bp) 6325
- Total vector size (bp) 6649
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Vector typeRetroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmall Ubiquitin Like Modifier 2
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Alt nameSUMO-2
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SpeciesH. sapiens (human)
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GenBank IDNM_006937.4 NM_006937.4
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Entrez GeneSUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
- Promoter PGK promoter
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Tag
/ Fusion Protein
- 6XHIS (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CCCTTGAACCTCCTCGTTCGACC
- 3′ sequencing primer GACAAATGGAAGTAGCACGTCTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCVpuro-6xHis_SUMO2 was a gift from Ji Luo (Addgene plasmid # 164938 ; http://n2t.net/addgene:164938 ; RRID:Addgene_164938) -
For your References section:
Oncogenesis driven by the Ras/Raf pathway requires the SUMO E2 ligase Ubc9. Yu B, Swatkoski S, Holly A, Lee LC, Giroux V, Lee CS, Hsu D, Smith JL, Yuen G, Yue J, Ann DK, Simpson RM, Creighton CJ, Figg WD, Gucek M, Luo J. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1724-33. doi: 10.1073/pnas.1415569112. Epub 2015 Mar 24. 10.1073/pnas.1415569112 PubMed 25805818