pTA231-p.Syn-Dendra2Hfx
(Plasmid
#164660)
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Purposeexpression of H. volcanii codon-optimized Dendra2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 164660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTA231-p.Syn
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Backbone manufacturerBerkemer et al., 2020, Identification of RNA 3´ ends and termination sites in Haloferax volcanii
- Backbone size w/o insert (bp) 7765
- Total vector size (bp) 8458
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Vector typearchaeal expression
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Selectable markersmarker for H. volcanii: trpA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDendra2Hfx
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Alt nameH. volcanii codon-optimized Dendra2
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SpeciesSynthetic
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Insert Size (bp)693
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Mutationcodon-optimization for Haloferax volcanii
- Promoter p.Syn
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDendra2Hfx ordered from GeneArt for synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTA231-p.Syn-Dendra2Hfx was a gift from Anita Marchfelder (Addgene plasmid # 164660 ; http://n2t.net/addgene:164660 ; RRID:Addgene_164660) -
For your References section:
Establishing Live-Cell Single-Molecule Localization Microscopy Imaging and Single-Particle Tracking in the Archaeon Haloferax volcanii. Turkowyd B, Schreiber S, Wortz J, Segal ES, Mevarech M, Duggin IG, Marchfelder A, Endesfelder U. Front Microbiol. 2020 Nov 20;11:583010. doi: 10.3389/fmicb.2020.583010. eCollection 2020. 10.3389/fmicb.2020.583010 PubMed 33329447