pAAV-hSyn-DIO-hMECP2-cycle3GFP
(Plasmid
#163706)
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PurposepAAV plasmid for Cre-dependent expression of human MECP2 fused with cycle3 GFP under Syn promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehMECP2-cycle3GFP
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SpeciesH. sapiens (human); Aequorea victoria
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Entrez GeneMECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
- Promoter hSyn
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GFP-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byhMecp2-cycle3GFP is from Huda Zoghbi (Addgene plasmid # 48078)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-DIO-hMECP2-cycle3GFP was a gift from Michisuke Yuzaki (Addgene plasmid # 163706 ; http://n2t.net/addgene:163706 ; RRID:Addgene_163706) -
For your References section:
MeCP2 Levels Regulate the 3D Structure of Heterochromatic Foci in Mouse Neurons. Ito-Ishida A, Baker SA, Sillitoe RV, Sun Y, Zhou J, Ono Y, Iwakiri J, Yuzaki M, Zoghbi HY. J Neurosci. 2020 Nov 4;40(45):8746-8766. doi: 10.1523/JNEUROSCI.1281-19.2020. Epub 2020 Oct 12. 10.1523/JNEUROSCI.1281-19.2020 PubMed 33046553