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Addgene

pAAV-Ef1a-fDIO-mCherryP2ACre
(Plasmid #161773)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161773 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    AAV
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 7411
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry-P2A-Cre
  • Species
    Aequorea victoria, bacteriophage P1
  • Insert Size (bp)
    1805
  • Promoter Ef1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ASCI (destroyed during cloning)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAAV-Ef1a-fDIO EYFP (Plasmid #55641)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-fDIO-mCherryP2ACre was a gift from Baoji Xu (Addgene plasmid # 161773 ; http://n2t.net/addgene:161773 ; RRID:Addgene_161773)
  • For your References section:

    TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits. An JJ, Kinney CE, Tan JW, Liao GY, Kremer EJ, Xu B. Nat Commun. 2020 Apr 7;11(1):1729. doi: 10.1038/s41467-020-15537-w. 10.1038/s41467-020-15537-w PubMed 32265438