-
PurposeFlp-Dependent mCherry and Cre expression
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 161773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV
-
Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 7411
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namemCherry-P2A-Cre
-
SpeciesAequorea victoria, bacteriophage P1
-
Insert Size (bp)1805
- Promoter Ef1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ASCI (destroyed during cloning)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypAAV-Ef1a-fDIO EYFP (Plasmid #55641)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-Ef1a-fDIO-mCherryP2ACre was a gift from Baoji Xu (Addgene plasmid # 161773 ; http://n2t.net/addgene:161773 ; RRID:Addgene_161773) -
For your References section:
TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits. An JJ, Kinney CE, Tan JW, Liao GY, Kremer EJ, Xu B. Nat Commun. 2020 Apr 7;11(1):1729. doi: 10.1038/s41467-020-15537-w. 10.1038/s41467-020-15537-w PubMed 32265438