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Addgene

pLV(cmv)-NEO-FSF-tdTomato Cre reporter
(Plasmid #160551)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160551 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8102
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (destroyed during cloning)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid may be particularly prone to recombination; please screen multiple colonies before selecting a clone to perform downstream experiments

SFS in paper should be FSF

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV(cmv)-NEO-FSF-tdTomato Cre reporter was a gift from Marc Vooijs (Addgene plasmid # 160551 ; http://n2t.net/addgene:160551 ; RRID:Addgene_160551)
  • For your References section:

    A lineage-tracing tool to map the fate of hypoxic tumour cells. Vermeer JAF, Ient J, Markelc B, Kaeppler J, Barbeau LMO, Groot AJ, Muschel RJ, Vooijs MA. Dis Model Mech. 2020 Jul 30;13(7). pii: dmm.044768. doi: 10.1242/dmm.044768. 10.1242/dmm.044768 PubMed 32571767
Commonly requested with: