pLV(cmv)-NEO-FSF-tdTomato Cre reporter
(Plasmid
#160551)
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Purposelabeling hypoxic cells, Cre reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 160551 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 8102
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdTomato
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site KpnI (destroyed during cloning)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid may be particularly prone to recombination; please screen multiple colonies before selecting a clone to perform downstream experiments
SFS in paper should be FSF
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV(cmv)-NEO-FSF-tdTomato Cre reporter was a gift from Marc Vooijs (Addgene plasmid # 160551 ; http://n2t.net/addgene:160551 ; RRID:Addgene_160551) -
For your References section:
A lineage-tracing tool to map the fate of hypoxic tumour cells. Vermeer JAF, Ient J, Markelc B, Kaeppler J, Barbeau LMO, Groot AJ, Muschel RJ, Vooijs MA. Dis Model Mech. 2020 Jul 30;13(7). pii: dmm.044768. doi: 10.1242/dmm.044768. 10.1242/dmm.044768 PubMed 32571767