pCVD313
(Plasmid
#159771)
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PurposeExpression vector for mutated Fc-fused ACE2(740)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 159771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFUSE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACE2
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SpeciesH. sapiens (human)
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MutationK31F/N33D/H34S/E35Q/H345L
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Entrez GeneACE2 (a.k.a. ACEH)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD313 was a gift from Jim Wells (Addgene plasmid # 159771 ; http://n2t.net/addgene:159771 ; RRID:Addgene_159771) -
For your References section:
Engineered ACE2 receptor traps potently neutralize SARS-CoV-2. Glasgow A, Glasgow J, Limonta D, Solomon P, Lui I, Zhang Y, Nix MA, Rettko NJ, Zha S, Yamin R, Kao K, Rosenberg OS, Ravetch JV, Wiita AP, Leung KK, Lim SA, Zhou XX, Hobman TC, Kortemme T, Wells JA. Proc Natl Acad Sci U S A. 2020 Nov 10;117(45):28046-28055. doi: 10.1073/pnas.2016093117. Epub 2020 Oct 22. 10.1073/pnas.2016093117 PubMed 33093202