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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLOVE
- Backbone size w/o insert (bp) 8263
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsExpand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namev-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
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Alt nameMycn, Nmyc, N-myc
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SpeciesM. musculus (mouse)
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Insert Size (bp)2500
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MutationThe recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Nmyc is ~9307 bp.
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Entrez GeneMycn (a.k.a. N-myc, Nmyc, Nmyc-1, Nmyc1, bHLHe37, c-nmyc)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer V5 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLOVE-Nmyc was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15951 ; http://n2t.net/addgene:15951 ; RRID:Addgene_15951) -
For your References section:
Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358