pF(UG) CamKII sf iGluSnFR
(Plasmid
#158769)
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PurposeiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW (#14883)
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiGluSnFR
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SpeciesM. musculus (mouse)
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MutationAdditional ER and Golgi export sequences
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In-fusion cloning was used for constuction of this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pF(UG) CamKII sf iGluSnFR was a gift from Edwin Chapman (Addgene plasmid # 158769 ; http://n2t.net/addgene:158769 ; RRID:Addgene_158769) -
For your References section:
Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. Vevea JD, Chapman ER. eLife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469. 10.7554/eLife.56469 PubMed 32515733