MAC-BSG
(Plasmid
#158383)
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PurposeMAC-tagged gene expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 158383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backboneMAC-tag-C (#108077)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBSG
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Alt nameCD147
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SpeciesH. sapiens (human)
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Entrez GeneBSG (a.k.a. 5F7, CD147, EMMPRIN, EMPRIN, HAb18G, OK, TCSF)
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Tag
/ Fusion Protein
- MAC-tag (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC-BSG was a gift from Markku Varjosalo (Addgene plasmid # 158383 ; http://n2t.net/addgene:158383 ; RRID:Addgene_158383) -
For your References section:
Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks. Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M. Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 10.1038/s41596-020-0365-x. 10.1038/s41596-020-0365-x PubMed 32778839