pCAG-HA-LMW-FGF2
(Plasmid
#157663)
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PurposeExpresses FGF2 in mammalian cell
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG-HA-puro
- Backbone size w/o insert (bp) 6377
- Total vector size (bp) 6842
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFibroblast growth factor 2
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Alt nameFGF2
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SpeciesH. sapiens (human)
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Insert Size (bp)465
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Mutationdeleted amino acid 1-134
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GenBank ID
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Entrez GeneFGF2 (a.k.a. BFGF, FGF-2, FGFB, HBGF-2)
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Tag
/ Fusion Protein
- HA tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-HA-LMW-FGF2 was a gift from Byung Il Lee (Addgene plasmid # 157663 ; http://n2t.net/addgene:157663 ; RRID:Addgene_157663) -
For your References section:
Regulation of mRNA export through API5 and nuclear FGF2 interaction. Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI. Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335. 10.1093/nar/gkaa335 PubMed 32383752