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Addgene

pHis-LMW_FGF2
(Plasmid #157657)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157657 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pHis
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5834
  • Modifications to backbone
    modified version of pET-28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fibroblast growth factor 2
  • Alt name
    FGF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    465
  • Mutation
    changed Cystein 211,229 to serine, deleted amino acids 1-134
  • GenBank ID
  • Entrez Gene
    FGF2 (a.k.a. BFGF, FGF-2, FGFB, HBGF-2)
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site E.coRI (unknown if destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis-LMW_FGF2 was a gift from Byung Il Lee (Addgene plasmid # 157657 ; http://n2t.net/addgene:157657 ; RRID:Addgene_157657)
  • For your References section:

    Regulation of mRNA export through API5 and nuclear FGF2 interaction. Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI. Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335. 10.1093/nar/gkaa335 PubMed 32383752