pHis-LMW_FGF2
(Plasmid
#157657)
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PurposeExpresses FGF2 in E.coli cell
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHis
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 5834
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Modifications to backbonemodified version of pET-28
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFibroblast growth factor 2
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Alt nameFGF2
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SpeciesH. sapiens (human)
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Insert Size (bp)465
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Mutationchanged Cystein 211,229 to serine, deleted amino acids 1-134
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GenBank ID
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Entrez GeneFGF2 (a.k.a. BFGF, FGF-2, FGFB, HBGF-2)
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site E.coRI (unknown if destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHis-LMW_FGF2 was a gift from Byung Il Lee (Addgene plasmid # 157657 ; http://n2t.net/addgene:157657 ; RRID:Addgene_157657) -
For your References section:
Regulation of mRNA export through API5 and nuclear FGF2 interaction. Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI. Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335. 10.1093/nar/gkaa335 PubMed 32383752