mChT pcDNA3
(Plasmid
#15765)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehemicholinium-3-sensitive choline transporter
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Alt nameChT
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SpeciesM. musculus (mouse)
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Insert Size (bp)1740
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Entrez GeneSlc5a7 (a.k.a. CHT1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mChT pcDNA3 was a gift from Randy Blakely (Addgene plasmid # 15765 ; http://n2t.net/addgene:15765 ; RRID:Addgene_15765) -
For your References section:
Molecular cloning and characterization of a murine hemicholinium-3-sensitive choline transporter. Apparsundaram S, Ferguson SM, Blakely RD. Biochem Soc Trans. 2001 Nov . 29(Pt 6):711-6. 10.1042/BST0290711 PubMed 11709061
Map uploaded by the depositor.
