pENTR1A hu_ncOGT WT
(Plasmid
#154281)
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PurposeEntry vector encoding non-tagged O-GlcNAc Transferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR1A
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Backbone manufacturerCampeau et al. PLOS One 2009
- Backbone size w/o insert (bp) 2300
- Total vector size (bp) 5384
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Vector typeGateway Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameO-GlcNAc Transferase
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Alt nameOGT
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SpeciesH. sapiens (human)
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Insert Size (bp)3111
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MutationWT OGT
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GenBank IDNM_181673.3
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Entrez GeneOGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
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Tags
/ Fusion Proteins
- mKate2 (N terminal on insert)
- 2x FLAG (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.10.22.351288 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR1A hu_ncOGT WT was a gift from Suzanne Walker (Addgene plasmid # 154281 ; http://n2t.net/addgene:154281 ; RRID:Addgene_154281) -
For your References section:
Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase. Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara NE, Gray NS, Paulo JA, Walker S. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 10.1073/pnas.2016778118. 10.1073/pnas.2016778118 PubMed 33419956