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PurposeBacterial expression of MMLV reverse transcriptase
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET-19b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMMLV Reverse Transcriptase
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SpeciesMMLV
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Tag
/ Fusion Protein
- 10x His (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid made by Alex Brown in Philippa Marrack's lab at National Jewish Health.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-19b_MMLV-RT was a gift from Drew Endy & Philippa Marrack (Addgene plasmid # 153312 ; http://n2t.net/addgene:153312 ; RRID:Addgene_153312)