pLIA (CC#1)
(Plasmid #15129)

• Purpose: (Empty Backbone)
• Depositing Lab: Connie Cepko
• Publication: Bao et al J Neurosci. 1997 Feb 15. 17(4):1425-34.

Backbone

• Vector backbone: pLIA
• Backbone manufacturer: Cepko Lab
• Backbone size (bp): 6500
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • IRES-PLAP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A replication-incompetent pLIA retroviral vector, based on the pBABE vector. In addition to the MMLV long terminal repeats, it also contains an internal ribosomal entry site (IRES) sequence derived from the encephalomyocarditis virus, in which the eleventh ATG of the IRES was fused in frame to the human placental alkaline phosphatase gene (PLAP).

pLIA has a cloning site for expressing exogenous genes under the transcriptional control of the LTR. The cloning site contains NotI, SfiI, SnaBI, and EcoRI.