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PurposeExpresses human ACE2 driven by the K18 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 149449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepSEAP
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Backbone manufacturerTropix, Bedford, MA
- Backbone size w/o insert (bp) 7114
- Total vector size (bp) 9532
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Modifications to backboneThe vector includes 5' and 3' genomic regions of the human K18 gene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAngiotensin-converting enzyme 2
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SpeciesH. sapiens (human)
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Insert Size (bp)2418
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter K18
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The vector includes 5' and 3' genomic regions of the human K18 gene, necessary for driving high-level epithelial-cell-specific expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK18-hACE2 was a gift from Paul McCray (Addgene plasmid # 149449 ; http://n2t.net/addgene:149449 ; RRID:Addgene_149449) -
For your References section:
Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus. McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M, Manzel L, Shi L, Netland J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S. J Virol. 2007 Jan;81(2):813-21. doi: 10.1128/JVI.02012-06. Epub 2006 Nov 1. 10.1128/JVI.02012-06 PubMed 17079315