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Addgene

hGip-R
(Plasmid #14942)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14942 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gip-R
  • Alt name
    Glucose-dependent insulinotropic polypeptide receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • Mutation
    Short form of the receptor
  • Entrez Gene
    GIPR (a.k.a. PGQTL2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Short form of the receptor; HindIII-AccI fragment of the long form plus AccI-XhoI fragment of pGIPR-17 in HindIII-XhoI of pcDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hGip-R was a gift from Bernard Thorens (Addgene plasmid # 14942 ; http://n2t.net/addgene:14942 ; RRID:Addgene_14942)
  • For your References section:

    Cloning, functional expression, and chromosomal localization of the human pancreatic islet glucose-dependent insulinotropic polypeptide receptor. Gremlich S, Porret A, Hani EH, Cherif D, Vionnet N, Froguel P, Thorens B. Diabetes. 1995 Oct . 44(10):1202-8. 10.2337/diabetes.44.10.1202 PubMed 7556958