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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGip-R
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Alt nameGlucose-dependent insulinotropic polypeptide receptor
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SpeciesH. sapiens (human)
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Insert Size (bp)3200
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MutationShort form of the receptor
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Entrez GeneGIPR (a.k.a. PGQTL2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Short form of the receptor; HindIII-AccI fragment of the long form plus AccI-XhoI fragment of pGIPR-17 in HindIII-XhoI of pcDNA3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hGip-R was a gift from Bernard Thorens (Addgene plasmid # 14942 ; http://n2t.net/addgene:14942 ; RRID:Addgene_14942) -
For your References section:
Cloning, functional expression, and chromosomal localization of the human pancreatic islet glucose-dependent insulinotropic polypeptide receptor. Gremlich S, Porret A, Hani EH, Cherif D, Vionnet N, Froguel P, Thorens B. Diabetes. 1995 Oct . 44(10):1202-8. 10.2337/diabetes.44.10.1202 PubMed 7556958